In the Tobacco Experimental Station at San Juan and Martinez, Pinar del Rio province, it was done from 1976 to 1980 an experiment about different doses of organic manure and limestone material on bottom of mineral fertilizers, forming a total of 12 treatments, of which the witness was not applied fertilizer or calcium carbonate, using the dark tobacco Criollo variety, grown in the sun. The water requirements of the crop were met by irrigation, which was made taking into consideration the development stages of tobacco and climatic conditions.

In agreement with the results obtained, it was proved the great effectiveness of mineral fertilizers and their use in combination with organic manure. The highest yield in higher grades of tobacco leaves was obtained with the application of 40 t / ha of organic manure on a background of fertilizer N-100 P-80, K-170, Mg-30, showed up to get 71-77% of higher classes.
From the economic point of view the best treatment was that applied 20 t / ha of organic manure on a background of mineral N-100, P-80, K-170, Mg-30, without liming, providing a 21% profitable. The organic manure does not exert significant influence on yields, but in does on obtain a greater percentage of higher grades of leaves.